本文轉載自原廠電子報: Creative Bioarray | dda.creative-bioarray.org
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Of many important features of drug candidates, permeability is an essential parameter to influence
the absorption and distribution of a drug. To test permeability, the use of in vivo or in situ animal
models raises unavoidable ethical concerns and presents a difficulty when a high-throughput screening
test is needed. Therefore, in vitro studies have been increasingly expected to evaluate drug permeability. |
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Creative Bioarray provides in vitro permeability assay services to determine intestinal, blood-brain
barrier (BBB) penetration or vascular permeability. Our experts are experienced working with permeability
models. We ensure consistent and high-quality data with a fast turnaround time. |
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In vitro intestinal permeability models |
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Creative Bioarray provides a set of monolayer
barrier permeability systems that can be time saving and cost-effective for your oral absorption,
bioavailability evaluation, drug transporter, and drug-drug interaction studies. |
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![Bioarray-20201013-InVitro-03](https://www.creative-bioarray.org/flyer/20201013-InVitro-03.png) |
Caco-2 epithelial monolayer barrier |
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![Bioarray-20201013-InVitro-03](https://www.creative-bioarray.org/flyer/20201013-InVitro-03.png) |
P-gp overexpressed Caco-2 epithelial monolayer barrier |
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![Bioarray-20201013-InVitro-03](https://www.creative-bioarray.org/flyer/20201013-InVitro-03.png) |
Transporter silenced Caco-2 (BCRP, MDR1) epithelial monolayer barrier |
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![Bioarray-20201013-InVitro-03](https://www.creative-bioarray.org/flyer/20201013-InVitro-03.png) |
Intestinal epithelial tight junction barrier (T84, HT29, Caco-2, and HCT116) |
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![Bioarray-20201013-InVitro-03](https://www.creative-bioarray.org/flyer/20201013-InVitro-03.png) |
Mucus-producing intestinal epithelial monolayer barrier (HT29- MTX) |
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![Bioarray-20201013-InVitro-03](https://www.creative-bioarray.org/flyer/20201013-InVitro-03.png) |
MDCK/MDR1 monolayer barrier |
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Ex vitro intestinal permeability models |
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Creative Bioarray offers an ex vivo permeability
assay using intestinal sacs to measure overall intestinal integrity and comparative transport of
a specific molecule. The intestinal sacs will provide a way to calculate the apparent permeability
of a molecule across the intestinal barrier or to examine regional epithelial barrier dysfunction
using fluorescent-labeled dextrans. |
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In vitro blood-brain barrier permeability models |
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To expedite brain research and the R&D of novel
drugs for various neurological diseases, we have established different in vitro BBB models. Our
novel models use only primary human cells and include four key BBB cells: astrocytes, pericytes,
brain microvascular endothelial cells (BMECs), and neurons. |
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![Bioarray-20201013-InVitro-03](https://www.creative-bioarray.org/flyer/20201013-InVitro-03.png) |
Monolayer BBB models (BMECs) |
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![Bioarray-20201013-InVitro-03](https://www.creative-bioarray.org/flyer/20201013-InVitro-03.png) |
Co-culture BBB models (BMECs–pericytes–astrocytes) |
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![Bioarray-20201013-InVitro-03](https://www.creative-bioarray.org/flyer/20201013-InVitro-03.png) |
Microfluidic models |
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With decades of operational experience, Creative Bioarray can provide the
following analytical services based on our permeability models: |
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![Bioarray-20201013-InVitro-04](https://www.creative-bioarray.org/flyer/20201013-InVitro-04.png) |
Prioritize candidates for the possibility of adverse effects |
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![Bioarray-20201013-InVitro-04](https://www.creative-bioarray.org/flyer/20201013-InVitro-04.png) |
Predict various permeability processes (paracellular, transcellular) |
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![Bioarray-20201013-InVitro-04](https://www.creative-bioarray.org/flyer/20201013-InVitro-04.png) |
Estimate different intracellular concentrations (membrane, cytosol, lysosome) |
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![Bioarray-20201013-InVitro-04](https://www.creative-bioarray.org/flyer/20201013-InVitro-04.png) |
Assess the impact of experimental variability on the predicted outcomes |
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![Bioarray-20201013-InVitro-04](https://www.creative-bioarray.org/flyer/20201013-InVitro-04.png) |
Analysis of measured in vitro permeability data |
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